Abstract #2759

Evaluation of the Early Metabolic Response Induced by 5-Fluorouracil on Mouse Mammary Cancer Cells Under Fasting Conditions Using Hyperpolarized 13C-Labeled Pyruvate.

Hitoshi Kubo¹, Masafumi Harada¹, Takamasa Abe², Hiroshi Maezawa³, Hiromu Nishitani⁴

¹Department of Medical Imaging, University of Tokushima, Tokushima, Japan; ²SBD/MR Division, Oxford Instruments KK., Tokyo, Japan; ³Department of Radiation Physics, Engineering and Biology, University of Tokushima, Tokushima, Japan; ⁴Department of Radiology, University of Tokushima, Tokushima, Japan

The early metabolic response of pyruvate and lactate induced by 5-fluorouracil (5FU) on mouse mammary cancer cells under normal and fasting conditions was evaluated by NMR measurement using hyperpolarized 1-13C-pyruvate and absorption spectroscopy. This study measured four combinations of nutritional status and treatment with 5FU. The rate constant of pyruvate-lactate metabolism was changed by the nutritional conditions without and with 5FU treatment. The results suggested that this technique allows the detection of the early metabolic response induced by an anticancer agent.