Meeting Banner
Abstract #2759

Evaluation of the Early Metabolic Response Induced by 5-Fluorouracil on Mouse Mammary Cancer Cells Under Fasting Conditions Using Hyperpolarized 13C-Labeled Pyruvate.

Hitoshi Kubo1, Masafumi Harada1, Takamasa Abe2, Hiroshi Maezawa3, Hiromu Nishitani4

1Department of Medical Imaging, University of Tokushima, Tokushima, Japan; 2SBD/MR Division, Oxford Instruments KK., Tokyo, Japan; 3Department of Radiation Physics, Engineering and Biology, University of Tokushima, Tokushima, Japan; 4Department of Radiology, University of Tokushima, Tokushima, Japan

The early metabolic response of pyruvate and lactate induced by 5-fluorouracil (5FU) on mouse mammary cancer cells under normal and fasting conditions was evaluated by NMR measurement using hyperpolarized 1-13C-pyruvate and absorption spectroscopy. This study measured four combinations of nutritional status and treatment with 5FU. The rate constant of pyruvate-lactate metabolism was changed by the nutritional conditions without and with 5FU treatment. The results suggested that this technique allows the detection of the early metabolic response induced by an anticancer agent.