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Abstract #2770

Magnetic Resonance Spectroscopy-Based CTP:choline-Phosphate Cytidylyltransferase Activity Measurement Technique

Christopher S. Ward1, Sabrina M. Ronen1

1Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States

CTP:choline-phosphate cytidylyltransferase (CCT) is the enzyme responsible for catalyzing the addition of CTP to phosphocholine to form CDP-choline, which is generally accepted to be the rate-limiting reaction in the formation of phosphatidylcholine. This study introduces a dynamic 31P MR-based means to measure the kinetics of the cytidylyltransferase-catalyzed reaction. The results suggest the potential of this technique to quantitatively assess modulations in CCT activity.