Abstract #3237

Proton MR Spectroscopy Measurements for Metabolomic Changes During Adipogenic Differentiation of Muscle Derived Stem Cells

Song I. Chun¹, Moo Young Jang¹, Sun Young Lee², Dong Hwa Kim¹, Jee Hyun Cho³, Jung Woog Shin¹, Young Il Yang², Chi Woong Mun^1,4

¹Biomedical Engineering, Inje University, Gimhae, Korea, Republic of; ²Pathology, Paik Hospital, Inje University, Busan, Korea, Republic of; ³Korea Basic Science Institute, Ochang, Korea, Republic of; ⁴UHRC, Inje University, Gimhae, Korea, Republic of

The purpose of this study is to measure and establish the metabolite change when the Muscle Derived Stem Cells (MDSCs) were differentiated into adipocyte using the 1H MR Spectroscopy. The experiments are classified four groups: Group1-Adipogenic Media, Group2- Papain digested fibrin gel, Group3-Papain digested MDSCs, Group4-Papain digested adipogenic MDSCs. The spectrum from each group has been acquired by utilizing vertical-bore 14.1T NMR/MRI with PRESS pulse sequence. Compare to spectrums of each group, we analyzed metabolite peaks newly formed during the differentiation of the MDSCs. In the results, we can observe that 1H MR spectral peak intensity increases at 0.89/1.24/1.9/2.48/3.0 ~3.1ppm after 14 days of differentiation from MDSCs into adipocyte. In this study, therefore we could observe the metabolite change along with MDSCs differentiation and found the potential possibilities of MRS to evaluate the differentiations of stem cell.