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Abstract #3278

In Vivo Detection of Rat Brain Metabolism Using Hyperpolarized Acetate

Mor Mishkovsky1,2, Arnaud Comment1,2, Rolf Gruetter1,3

1Laboratory for Functional and Metabolic Imaging , Ecole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland; 2Department of Radiology, Universit de Lausanne, Lausanne, Switzerland; 3Departments of Radiology, Universits de Lausanne et Genve, Lausanne and Genve, Switzerland

In vivo localized 13C MRS of a rat brain was performed in a 9.4T animal scanner after infusion of a hyperpolarized 1-13C and 13C2 sodium acetate solution. It is demonstrated that hyperpolarized 13C-labeled acetate rapidly enters the brain and its metabolism can be detected within the time window defined by the lifetime of the carboxyl carbon hyperpolarized spin state. We present a new sequence designed to transfer the carboxyl nuclear polarization onto the methyl carbon spin of 13C2 acetate in order to assign the metabolic peaks observed in vivo.