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Abstract #3277

Time Resolved Metabolic 13C MRS Using Hyperpolarised [1-13C]pyruvate in a Transgenic Mammary Cancer Model

Sadia Asghar Butt1, Lise Vejby Sgaard1, Mette Hauge Lauritzen1, Jan Henrik Ardenkjr-Larsen2, Lars H. Engelholm3, Susanne Holck4, Peter Magnusson1, Per keson1

1Danish Research Centre for Magnetic Resonance, Copenhagen University Hospital, Hvidovre, Denmark; 2GE Healthcare, Hillerd, Denmark; 3Bartholin Institute, Copenhagen Biocenter; 4Department of pathology, Copenhagen University Hospital Hvidovre


There is a need for monitoring of disease progression and treatment response in breast cancer using metabolic biomarkers. We have applied the technique of time resolved hyperpolarised 13C metabolic MRS to the transgenic mammary cancer mouse model, MMTV-PymT. We use measurements of the lactate dehydrogenase (LDH) rate constant reflecting the conversion of hyperpolarized [1-13C]pyruvate to [1-13C]lactate in vivo to extract information about the metabolic the status of the tissue Our initial results show that we are able to monitor the changes in LDH kinetics during tumour development and indicate that the enzyme activity correlate to disease progression.

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