Kayvan R. Keshari1, David M. Wilson, Albert
P. Chen2, Robert Bok, Peder E.Z. Larson, Simon Hu, Mark Van
Criekinge, Jeffrey M. Macdonald3, Daniel B. Vigneron, John
Kurhanewicz
1University of California, San
Francisco, San Francisco, Ca, United States; 2GE Healthcare; 3University
of North Carolina, Chapel Hill
In
this study, [2-13C]-fructose was hyperpolarized using the DNP
method and shown to have sufficiently long T1s (≈ 14 sec) and
polarizations (≈ 12%) for in vivo hyperpolarized 13C MRSI
studies. After injection of [2-13C]-fructose
in the TRAMP prostate cancer model, the resonance corresponding to the
composite β-fructofuranose and β-fructofuranose-6-phosphate was
higher in the regions of tumor as compared to the contralateral benign
prostate. The hemiketal C2 of fructose demonstrates the first non-carbonyl to
be hyperpolarized for use as a metabolic probe, providing the potential to
measure changes in carbohydrate metabolism that occur with human disease.
Keywords