Xing Chen1, Anke Henning1, Matteo Pavan1, Susanne Heinzer-Schweizer1, Marco Toigo2,3, Peter Bsiger1
1Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland; 2Institute of Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Switzerland; 3Exercise Physiology, Institute of Human Movement Sciences, ETH Zurich, Switzerland
13C MRS glycogen was detected by combined ISIS-localized NOE and proton decoupling in human calf muscle, with the ERETIC signal as a synthetic reference standard for quantification. Reproducibility tests were performed regarding the quantified signal intensities, SNR enhancement factors and ERETIC signal stability. In conclusion, the combination of NOE and decoupling can enhance the glycogen signal at a reproducible level and enables higher fitting and thus quantification reliability. As ERETIC signal stability proved to be unaffected by SNT enhancement, it is promising to use the ERETIC signal as reference for absolute quantification among different subjects and experiments.