Abstract #3350

ERETIC-Based Glycogen Quantification Using SNR-Enhanced and Localized 13C MRS

Xing Chen¹, Anke Henning¹, Matteo Pavan¹, Susanne Heinzer-Schweizer¹, Marco Toigo^2,3, Peter Bsiger¹

¹Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland; ²Institute of Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Switzerland; ³Exercise Physiology, Institute of Human Movement Sciences, ETH Zurich, Switzerland

13C MRS glycogen was detected by combined ISIS-localized NOE and proton decoupling in human calf muscle, with the ERETIC signal as a synthetic reference standard for quantification. Reproducibility tests were performed regarding the quantified signal intensities, SNR enhancement factors and ERETIC signal stability. In conclusion, the combination of NOE and decoupling can enhance the glycogen signal at a reproducible level and enables higher fitting and thus quantification reliability. As ERETIC signal stability proved to be unaffected by SNT enhancement, it is promising to use the ERETIC signal as reference for absolute quantification among different subjects and experiments.