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Abstract #3361

1H NMR Spectroscopic Studies on Human Seminal Plasma: A Probative Discriminate Function Analysis Classification Model

ashish gupta1, Abbas Ali Mahdi1, Kaleem Ahmad1, Kamla Kant Shukla1, S P. Jaiswar2, S N. Shankhwar3

1Department of Biochemistry, King Georges Medical University, Lucknow, UP, India; 2Department of Obstetric & Gynecology, King Georges Medical University, Lucknow, UP, India; 3Department of Urology, King Georges Medical University, Lucknow, UP, India


Quantitative analysis of seminal plasma (n=175) profile was carried out using 1H NMR spectroscopy and clinical symptoms were also observed in same samples with standard laboratory method. Multivariate discriminant function analysis (DFA) was carried out for the NMR observed metabolites and clinical symptoms data of the infertile and control cases, to find out important signature descriptors for classification. A new INFERTIX classification model was developed and proposed which is based on the results obtained from DFA for the different classes of infertile patients, with very high sensitivity and specificity values.

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