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Abstract #3390

Evaluation of Artificial Blood Substitutes by Quantitatively Measuring Multi-Organ Oxygen Using 19F MRI in a Rat Model

Siyuan Liu1,2, Sameer J. Shah2, Lisa J. Wilmes1, Vikram D. Kodibagkar3, Michael F. Wendland1, Christian C. Apfel2, Nola M. Hylton1, Harriet W. Hopf4, Ralph P. Mason3, Mark D. Rollins2

1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Anesthesia, University of California, San Francisco, San Francisco, CA, United States; 3Radiology, University of Texas at Southwestern, Dallas, TX, United States; 4Anesthesiology, University of Utah, Salt Lake City, UT, United States


Hemoglobin based oxygen carriers (HBOCs) are being developed to reduce blood transfusion, yet HBOCs efficacy on organ oxygenation remain unknown. We used 19F MRI to quantify tissue oxygen (ptO2) changes during isovolemic anemic hemodilution using high and low affinity HBOCs or colloid control at 30% and 100% inspired oxygen in a rat model. Although ptO2 significantly increased with 100% vs 30% oxygen under all conditions, differences in ptO2 between HBOCs or colloid were insignificant. Our results highlight the impact of supplemental oxygen, emphasize need for further HBOC research, and demonstrate the value of 19F MRI in quantifying resuscitation interventions.

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