Marie Poirier-Quinot1, Claire Wilhelm2,
Mohammed Derkaoui3, Jean-Christophe Ginefri1, Nathalie
Luciani2, Luc Darrasse1, Didier Letourneur3,
Florence Gazeau2, Catherine Le Visage3
1Imagerie par Rsonance Magntique
Mdicale et Multimodalits (UMR 8081 ), Univ Paris Sud, CNRS, Orsay, France; 2UMR
7057 CNRS/Univ Paris - Diderot, France; 3Inserm U698, CHU X. Bichat Paris, France
Polymeric
scaffolds, involved in tissue engineering, for cell seeded migration and
proliferation, are often extremely sensitive. Therefore 3D non-invasive
imaging methods are needed to study tissue-engineered constructs. This work
has demonstrated the efficiency of high resolution imaging, using a
superconducting surface coil at 1.5 T, with efficient medium and cellular
contrast agents, for 3D visualization of tissue-engineered constructs. The
labeled cell presence was quantified within the entire structure and their
spatial distribution was assessed along the privileged orientation of the
pores. According to these results, spatial distribution of cells is easily
monitored through the complex microstructure of scaffolds.
Keywords