Meeting Banner
Abstract #4173

Magnetic Resonance MicroImaging of Cell Migration in Porous Biomaterial Scaffolds Designed for Tissue Engineering

Marie Poirier-Quinot1, Claire Wilhelm2, Mohammed Derkaoui3, Jean-Christophe Ginefri1, Nathalie Luciani2, Luc Darrasse1, Didier Letourneur3, Florence Gazeau2, Catherine Le Visage3

1Imagerie par Rsonance Magntique Mdicale et Multimodalits (UMR 8081 ), Univ Paris Sud, CNRS, Orsay, France; 2UMR 7057 CNRS/Univ Paris - Diderot, France; 3Inserm U698, CHU X. Bichat Paris, France

Polymeric scaffolds, involved in tissue engineering, for cell seeded migration and proliferation, are often extremely sensitive. Therefore 3D non-invasive imaging methods are needed to study tissue-engineered constructs. This work has demonstrated the efficiency of high resolution imaging, using a superconducting surface coil at 1.5 T, with efficient medium and cellular contrast agents, for 3D visualization of tissue-engineered constructs. The labeled cell presence was quantified within the entire structure and their spatial distribution was assessed along the privileged orientation of the pores. According to these results, spatial distribution of cells is easily monitored through the complex microstructure of scaffolds.