MRI of Vascular Cells Labeled with SPIO-PLL Complexes for Heart Valve Tissue Engineering Studies

Paul A. Schornack¹, Sharan Ramaswamy

Noninvasive & nondestructive monitoring of the cellular function within the developing valvular tissue is a critical aspect of implant success. In-depth study on the longitudinal (temporal) position & migration patterns of cells during the tissue development process. This can be achieved through cellular MRI (cMRI) techniques such as with the labeling of cells with superparamagnetic iron oxide (SPIO) particles. Immediate goal Conduct efficient, non-toxic, endosomal uptake studies of SPIO particles in endothelial cells (ECs) & smooth muscle cells (SMCs)