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Abstract #4194

Characterization of MPIO Labeled Primary Murine Bone Marrow Derived Macrophages

Kevin S. Tang1, Erik M. Shapiro, 1,2

1Department of Biomedical Engineering, Yale University, New Haven, CT, United States; 2Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT, United States

Macrophages are key players in the innate immune response and important markers of local inflammation. Here, we evaluated the effects of MPIO labeling on macrophage functions: cytokine secretion, maintained phagocytosis, and cell migration. Labeling with MPIOs did not, on its own, stimulate the cells to produce TNF- and IL-12, two important inflammatory cytokines. Furthermore, MPIO labeling did not inhibit macrophages to secrete these cytokines upon activation with LPS. Fluorescence microscopy demonstrates the ability to continue phagocytosis after labeling. Lastly, transwell migration assays showed migration from both unlabeled and labeled macrophages, suggesting no effect on migratory ability by MPIOs.