Regional Difference in Mn Uptake and Retention in Mouse Brain

Abu-Bakar-Md-Ali Asad¹, Kai-Hsiang Chuang¹

We compared the difference in Mn uptake and retention rates at various brain regions after systemic administration of MnCl2. By estimating Mn concentration with the change in T1 relaxation rate (ΔR1), it was observed that different brain regions have considerable difference in the time-to-peak and bioelimination rate of Mn. The ΔR1-time course was fitted to a gamma variate model which showed reasonable fit and provides estimation of time-to-peak, peak value and half-life. The olfactory bulb reached the peak earliest and highest, while regions like thalamus reached peak at day 2. Olfactory bulb had fastest clearance with a half-life of 6.6 days, and cerebellum had longer half-life of 11.5 days. This method can allow better estimate of the uptake and retention and can be used to maximize tissue contrasts or applied to studying of transporter mechanism in animal models.