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Abstract #4512

Monitoring Demyelination in a Cuprizone Mouse Model with Longitudinal and Quantitative MRI Measurements

Jonathan D. Thiessen1, Yanbo Zhang2, Handi Zhang2, Lingyan Wang2, Richard Buist3, Jiming Kong4, Xin-Min Li2, Melanie Martin1,5

1Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada; 2Psychiatry, University of Manitoba; 3Radiology, University of Manitoba; 4Human Anatomy and Cell Science, University of Manitoba; 5Physics, University of Winnipeg


Magnetic resonance imaging methods capable of quantifying changes due to demyelination can improve both the diagnosis and understanding of white matter diseases such as multiple sclerosis. T2-weighted and magnetization transfer images (MTI) were acquired weekly in control (n=4) and cuprizone-fed mice (n=4) from 2 to 6 weeks of treatment. Diffusion tensor imaging, quantitative MTI, high-resolution T2-weighted imaging, and histopathology were used to analyze ex vivo tissue. All in vivo methods showed significant differences longitudinally in the corpus callosum of the cuprizone-fed mouse. All in vivo and ex vivo methods showed significant differences in the corpus callosum between groups.

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