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Abstract #4580

In Vivo Imaging of Mouse Pancreas Utilizing Ultra High Field of 14T and Manganese Enhanced MRI

Riikka J. Immonen1, Smaragda Lamprianou2, Laurent Lecomte1, Laurent Vinet2, Paolo Meda2, Rolf Gruetter1,3

1Laboratory for functional and metabolic imaging, Ecole Polytechnique Fdrale de Lausanne, Lausanne, CH-1015, Switzerland; 2Department of Cell Physiology and Metabolism, University of Geneva, Geneva, CH-1210, Switzerland; 3Department of Radiology, University of Geneva and Lausanne, Geneva/Lausanne, CH-1210/CH-1015, Switzerland

In vivo imaging of pancreas with high resolution is challenging. In diabetes pancreatic βcell mass and function are gradually lost. MRI methodology to monitor this in vivo is needed. We utilized 14.1T and manganese enhanced MRI to image the mouse gland in vivo, and to examine the changes in manganese signal during βcell stimulation by glucose. In multi-slice images with 50*50*300μm resolution, acquired <20min, we were able to distinguish pancreatic main vessels and exocrine ducts, and Mn highlighted spots, possibly pancreatic islets. We also demonstrated with T1-wt inversion recovery, lower resolution technique, the manganese enhancement before and after glucose stimulus.