Riikka J.
Immonen1, Smaragda Lamprianou2, Laurent Lecomte1,
Laurent Vinet2, Paolo Meda2, Rolf Gruetter1,3
1Laboratory for functional and metabolic
imaging, Ecole Polytechnique Fdrale de Lausanne, Lausanne, CH-1015,
Switzerland; 2Department of Cell Physiology and Metabolism,
University of Geneva, Geneva, CH-1210, Switzerland; 3Department of
Radiology, University of Geneva and Lausanne, Geneva/Lausanne,
CH-1210/CH-1015, Switzerland
In
vivo imaging of pancreas with high resolution is challenging. In diabetes
pancreatic βcell mass and function are gradually lost. MRI methodology
to monitor this in vivo is needed. We utilized 14.1T and manganese enhanced
MRI to image the mouse gland in vivo, and to examine the changes in manganese
signal during βcell stimulation by glucose. In multi-slice images with
50*50*300μm resolution, acquired <20min, we were able to distinguish
pancreatic main vessels and exocrine ducts, and Mn highlighted spots,
possibly pancreatic islets. We also demonstrated with T1-wt inversion
recovery, lower resolution technique, the manganese enhancement before and
after glucose stimulus.
Keywords