Correlation Between TCho Peak at H-MRS and Gene Expression of Choline Kinases and Transmembrane Choline Transporters: An Experimental Study on a Rodent Rhabdomyosarcoma Model Using a Standard 3T Clinical Imager

Denis Rommel¹, Frank Peeters², Jorge Abarca-Quinones², Christine De Saeger³, Isabelle Leclercq³, Thierry Duprez¹

We investigated which from transmembrane choline transporters and choline kinases had the most prominent role in the elevation of the tCho peak at H-MRS using a rodent rabdomyosarcoma model. tCho peak was quantified as the area under the curve at 3.2 ppm obtained on a 3T clinical system, and gene expression was quantified by PCR after reverse transcription into cDNA using standard ΔCt calculations with reference to RPL19 gene expression. The prominence of the choline kinase α expression versus that of the transmembrane choline transporters was strongly suggested by the statistical analysis.