Adrienne E. Campbell1,2, Anthony N. Price3, Jack A. Wells1, Roger J. Ordidge2, Mark F. Lythgoe1
1Centre for Advanced Biomedical Imaging, Division of Medicine & Institute of Child Health, University College London, London, United Kingdom; 2Department of Medical Physics & Bioengineering, University College London, London, United Kingdom; 3Robert Steiner MRI Unit, Imaging Science Department, Hammersmith Hostpital, Imperial College London, London, United Kingdom
A time-efficient arterial spin labelling sequence was implemented to measure perfusion in the mouse heart. Data acquisition was improved using a data logger for additional cardiac gating and the generation of objective criteria for rejection of respiration corrupted images. A validation study was performed on 8 CD-1s to analyse the sources of variability in this technique, and to quantify the repeatability of this technique for group comparisons and for longitudinal studies. It was found that between animal variations were larger than technique variations and that achievable changes in perfusion were required for observation of group differences, beyond technique error.