Benjamin Marty1, Benoit Larrat1, Caroline Robic2, Mathieu Pernot3, Mickael Tanter3, Marc Port2, Philippe Robert2, Denis Le Bihan1, Franck Lethimonnier1, Sbastien Mriaux1
Lately, many studies have shown the ability to disrupt transiently the Blood Brain Barrier using ultrasound sonication of intravascular microbubbles. However, BBB opening mechanism is not properly known, especially the maximum gap reachable in safe conditions. We used contrast agents of different size to study the closure mechanism of BBB and the maximum molecule size able to penetrate cerebral tissues. BBB opening was obtained in vivo for contrast agents of sizes up to 65nm and its closure exhibits a fast initial closure of largest gaps. Those findings are valuable information in the framework of high molecular weight drug delivery.