Mor Mishkovsky1,2,
Arnaud Comment1,2, Rolf Gruetter1,3
1Laboratory for
Functional & Metabolic Imaging , Ecole Polytechnique Fdrale de
Lausanne, Lausanne, Switzerland; 2Department of Radiology,
Universit de Lausanne, Lausanne, Switzerland; 3Department of
Radiology, Universits de Lausanne et de Genve, Lausanne & Genve,
Switzerland
Acetate brain metabolism was studied in vivo in rats following the infusion of hyperpolarized 1-13C and 13C2 sodium acetate solutions, leading to the first direct observation of the brain TCA cycle intermediate 2-oxoglutarate (2OG). The observation of 2OG and the lack of glutamate (Glu) signal imply that the reactions leading to 13C-label incorporation into Glu are operating, in the glial compartment in vivo, at a rate much lower than that of transaminase. We deduced from this observation that transport across the inner mitochondria membrane is rate limiting.
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