Abstract #0713

Dependence of CEST Effect from Amine Protons of Glutamate on pH

Anup Singh¹, Kejia Cai¹, Mohammad Haris¹, Joel H. Greenberg², Hari Hariharan¹, Ravinder Reddy¹

¹CMROI, Department of Radiology, University of Pennsylvania, Philadelphia, PA, USA; ²Department of Neurology, University of Pennsylvania, Philadelphia, PA, USA

Dependence of CEST effect from amine protons of Glutamate (GluCEST) on pH is demonstrated using phantom and in-vivo rat data. While GluCEST has a non-linear dependence on pH from 1-8 it appear to vary linearly over a physiologically relevant pH changes between 6 and 7.4. In the ipsilateral side of middle cerebral artery occlusion (MCAO) model of stroke in rat brain, GluCEST is almost doubled at 4.5 hrs after occlusion compared to contralateral side.