Abstract #0736

Direct Multi-Tissue Assessment of In Vivo Postprandial Lipid Handling in ZDF Rats using Localized 1H-[13C] MRS

Richard Jonkers¹, Luc van Loon², Klaas Nicolay¹, Jeanine Prompers¹

¹Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands; ²Department of Human Movement Sciences, Maastricht University Medical Centre+, Maastricht, Netherlands

Insulin resistance and type 2 diabetes are associated with excessive lipid accumulation in liver and muscle. In this study, we investigated in vivo lipid handling in liver and muscle tissue of pre-diabetic and diabetic ZDF rats using localized ¹H-[¹³C] MRS after administration of ¹³C-labeled lipids. In liver, lipid uptake was increased in both pre-diabetic and diabetic rats compared with controls. Lipid uptake in muscle was likewise increased in diabetic rats, but in contrast it was lower in pre-diabetic rats compared with controls. Whereas lipid utilization was largely unaffected in liver, it was impaired in muscle of pre-diabetic and diabetic rats.