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Abstract #0853

13C-Labelling & Non-Invasive Detection of Glutathione in Human Liver

Peter Edward Thelwall1, Fiona Elizabeth Smith1, Matthew Clemence2, Kieren G. Hollingsworth1, Roy Taylor1, Michael P. Gamcsik3

1Newcastle Magnetic Resonance Centre, Newcastle University, Newcastle upon Tyne, Tyne & Wear, United Kingdom; 2Philips Healthcare - Clinical Science, Guildford, United Kingdom; 3Joint Department of Biomedical Engineering, University of North Carolina / NC State University, United States

Glutathione is a tripeptide that is a central component of cellular defences against oxidative stress. We have shown that a 13C label can be introduced into glutathione in humans by oral administration of [2-13C]-glycine, where this amino acid is incorporated into glutathione. We have shown that 13C-labelled glutathione can be detected by 1H-decoupled 13C spectroscopy in human liver, allowing measurements of glutathione concentration and synthesis rate and providing an insight into cellular defences against oxidative stress.