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Abstract #1466

Histological Confirmation of Aplysia Californica Neuron Structure Observed using MR Microscopy

Choong H. Lee1,2, Jeremy Joseph Flint3,4, Michael Fey5, Franck Vincent5, Stephen Blackband4,6

1Electrical Engineering, University of Florida, Gainesville, Fl, United States; 2McKnight Brain Institute, Gainesville , Fl, United States; 3Neuroscience, University of Florida, Gainesville, Fl, United States; 4McKnight Brain Institute, Gainesville, Fl, United States; 5Bruker Biospin; 6National High Magnetic Field Laboratory, Tallahassee, Fl, United States

Although MR microscopy has visualized single cells, verification of the assignment of cellular compartments has not been performed in Aplysia californica neurons. Using a variety of stains, new microcoils, thin slices of fixed neurons, and the highest resolution MRM on Aplysia neurons reported to date, the assignment of the cytoplasmic and nuclear compartments and the plasma membrane is verified. Also, when collagenase is not used, many small cells are observed on the large neuron. These results impact future modeling approaches of MR signals in tissues.