Anna Pawlak1, Ryohei Ito1, Hirotada Fujii2, Hiroshi Hirata1
1Division of Bioengineering & Bioinformatics, Graduate School of Information Science & Technology, Hokkaido University, Sapporo, Hokkaido, Japan; 2Center for Medical Education, Sapporo Medical University, Sapporo, Hokkaido, Japan
The major advancement outlined in this study is the simultaneous visualization of two kinds of nitroxyl radicals using electron paramagnetic resonance (EPR) spectroscopy and imaging. We demonstrated the simultaneous imaging of 14N-labeled and 15N-labeled nitroxyl radicals with a 750-MHz CW-EPR imager. By using the characteristics of hyperfine structure of nitroxyl radicals, the distribution of isotopic nitroxyl radicals could be visualized simulaneously. We show the proof-of-concept experiments for simultaneous EPR imaging.