Abstract #1470

Simultaneous CW-EPR Imaging of Isotopic Nitroxyl Radicals

Anna Pawlak¹, Ryohei Ito¹, Hirotada Fujii², Hiroshi Hirata¹

¹Division of Bioengineering & Bioinformatics, Graduate School of Information Science & Technology, Hokkaido University, Sapporo, Hokkaido, Japan; ²Center for Medical Education, Sapporo Medical University, Sapporo, Hokkaido, Japan

The major advancement outlined in this study is the simultaneous visualization of two kinds of nitroxyl radicals using electron paramagnetic resonance (EPR) spectroscopy and imaging. We demonstrated the simultaneous imaging of 14N-labeled and 15N-labeled nitroxyl radicals with a 750-MHz CW-EPR imager. By using the characteristics of hyperfine structure of nitroxyl radicals, the distribution of isotopic nitroxyl radicals could be visualized simulaneously. We show the proof-of-concept experiments for simultaneous EPR imaging.