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Abstract #1469

Detection of Blood-Brain Barrier Disruption in a Mouse Model of Transient Cerebral Ischemia by EPR Imaging

Hirotada G. Fujii1, Katsuya Kawanishi2, Hideo Sato-Akaba3, Miho Emoto1, Hiroshi Hirata4

1Center for Medical Education, Sapporo Medical Univeristy, Sapporo, Hokkaido, Japan; 2Health Sciences University of Hokkaido, Japan; 3Osaka University, Japan; 4Hokkaido University, Japan

In vivo EPR imaging provides a powerful method for the evaluation and visualization of oxidative stress, non-invasively. The purpose of this study was to examine the effect of oxidative stress on an ischemia-reperfusion (IR) model mouse brain with an improved EPR imaging system, capable of rapid field scanning. The results obtained in this study clearly show that through the use of blood-brain barrier (BBB)-permeable and BBB-impermeable nitroxide, improved EPR imaging can be used to asses BBB permeability in the wounded hemisphere of IR model mice. With three-dimensional surface-rendered images, the infarcted hemisphere of IR model mice can be visualized.