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Abstract #1655

Dual-Targeting of αvβ3 integrin & Galectin-1 Improves the Specificity of Paramagnetic, Fluorescent Liposome Association with Tumor Endothelium In Vivo

Ewelina Kluza1, Igor Jacobs1, Stefanie J. Hectors1, Kevin H. Mayo2, Arjan W. Griffioen3, Gustav J. Strijkers1, Klaas Nicolay1

1Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands; 2Department of Biochemistry, Molecular Biology & Biophysics, University of Minnesota, Minneapolis, United States; 3Angiogenesis Laboratory, Department of Medical Oncology, VU Medical Center, Amsterdam, Netherlands

Recently, we showed that paramagnetic liposomes, which are concurrently targeted to two cell surface markers give a strong enhancement in endothelial cell association in vitro. Here, we tested the hypothesis that dual-targeted liposomes also afford a more specific tumor endothelial association in vivo, using C57BL/6 mice bearing s.c. B16F10 tumors. Paramagnetic liposomes targeted to αvβ3-integrin and galectin-1 at the same time were compared to single-marker targeted versions. Tumor delivery was monitored with T1W-MRI and T1 mapping. Fluorescence microscopy confirmed that the dual-targeted liposomes afforded the highest targeting specificity, making this an attractive concept for improved MRI-based tumor angiogenesis imaging.