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Abstract #1708

Verification of Metabolite Peak Change During Chondrogenesis of Human Mesenchymal Stem Cells using Proton NMR

Moo-Young Jang1, So-Hee Park1, Jung-Woog Shin1,2, Chi-Woong Mun1,2

1Biomedical Engineering, Inje University, Gimhae, Gyeongnam, Korea, Republic of; 2UHRC, Inje University, Gimhae, Gyeongnam, Korea, Republic of

This paper is intended to investigate on metabolite changes of chondrogenic hMSCs using 1H NMR to confirm the differentiation. Chondrogenic hMSCs were resuspended to 0.9% saline D2O and was filled in 5mm NMR tube (2x106cells/500l). We confirmed differentiation by alcian blue staining. NMR spectrum data were acquired using 500MHz NMR spectrometer with the following parameters: PRESAT sequence, relaxation delay=20ms, 45 pulse, NT=512. We confirmed that peak intensities of 1H MR spectrum tend to increase at 1.33/1.48/1.49/2.94-2.97/3.23/3.56/ 3.78 ppm after the onset of chondrogenic differentiation from hMSCs. On the other hand, lipid and fatty acid peaks were reduced during the differentiation.