Su Xu1,2, Da Shi1,2, Steven Roys1,2, Alan McMillian1,2, Rao Gullapalli1,2, Rich Lovering3
1Diagnostic Radiology & Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States; 2Core for Translational Research in Imaging @ Maryland University of Maryland School ; 3Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD, United States
We assessed the potential of in vivo 1H MRS to monitor the intra-myocellular lipids in acute skeletal muscle injury in healthy and mdx mice (an animal model for human Duchenne muscular dystrophy). We found that mdx mice demonstrated very low intra-myocellular lipids level. However, injury caused a significant increase in intra-myocellular lipids in the mdx group only. Histology confirmed the changes quantified by the in vivo 1H MRS. We propose that in vivo 1H MRS is a sensitive measure of muscle injury with potential applicability in preclinical therapeutic screening studies in rodents.