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Abstract #3703

In Vivo Labelling of Xenografted B16 Melanoma Cells with a Thiol-Responsive Gd(III) Based MRI Contrast Agent

Eliana Gianolio1, Valeria Menchise2, Giuseppe Digilio3, Evelina Cittadino4, Carla Carrera4, Valeria Catanzaro4, Silvio Aime4

1Universit di Torino, Torino, Italy; 2CNR - IBB, Italy; 3Universit del Piemonte Orientale, Italy; 4Universit di Torino, Italy

Mammalian cells display a large number of reactive protein thiols on the extracellular side of the plasma membrane (Exofacial Protein Thiols, EPTs). These EPTs are quite reactive and can be chemically labeled by suitably designed MRI contrast agents. Compound Gd-DO3A-PDP can efficiently label B16 melanoma cells both ex vitro and in vivo (xenografted tumor) as a function of the levels of EPTs shown by cells.