Myriam Marianne Chaumeil1, Sarah Woods1, Robert M. Danforth1, Hikari Yoshihara1, Alessia Lodi1, Aaron Robinson2, Joanna J. Philips2, 3, Sabrina M. Ronen1
1Radiology, University of California, San Francisco, San Francisco, CA, United States; 2Neurological Surgery, University of California, San Francisco, San Francisco, CA, United States; 3Pathology, University of California, San Francisco, San Francisco, CA, United States
The mutational status of isocitrate dehydrogenase (IDH1) was assessed using dynamic 13C MRS of hyperpolarized (HP) α-ketoglutarate in two glioblastoma cells lines: U87 transduced with IDH1 wild-type (U87IDHwt) or IDH mutant (U87IDHmut). Following injection of HP α-KG, HP 2-hydroxyglutarate and HP glutamate were detected in U87IDHmut lysates. Only HP glutamate was visible in U87IDHwt lysates. Injection of HP α-KG in live perfused U87IDHwt cells resulted in dynamic HP glutamate build-up whereas no HP glutamate was detected in perfused U87IDHmut cells. This pioneer study demonstrates that HP α-KG can inform on IDH mutational status through the dynamic assessment of its metabolism.