2-Hydroxyglutarate in Gliomas with IDH Gene Mutation Using High Resolution 1H-NMR Spectroscopy of Tissue Extracts

H-M. Baek¹, I. Marin-Valencia¹, T. Mashimo¹, Z. Zhao¹, R. DeBerardinis¹, R. Bachoo¹, C. Malloy¹, ², E. Meher¹

This study represents, to our knowledge, the first In Vitro measurements of absolute quantification of 2HG levels in IDH1/2 mutated tumors using high resolution 1H-NMR spectroscopy. The levels of 2HG in this work had a range of 0.55 - 3.51 _μ mol/g from seven glioma samples with IDH1/2 mutation (e.g., 3 WHO grade II and 4 WHO grade III). In addition, Glu levels were found to be lower in IDH1/2 mutant tumors compared to IDH1/2 wild type tumors (P < 0.0001). This result may reflect that IDH mutations cause a decrease in Glu and/or _α -ketoglutarate production and an increase in 2HG.