In Vivo Absolute Quantification for Mouse Brain Tumor Using an Inductively Coupled Synthetic Signal Injection Method

Donghoon Lee¹, Kenneth Marro¹, Mark Mathis¹, Eric Shankland¹, Cecil Hayes¹, Stacey Hansen², James Olson²

We obtained robust estimates of 31P metabolite content in mouse brain tumor using a synthetic signal injection method and an optimized, 1H/31P dual tuned probe. 31P metabolite concentrations were determined for brain tumors in a genetically engineered mouse model of medulloblastoma. The dual tuned 1H/31P probe was composed of a half volume coil and a second coil to allow injection of pseudo signals. In addition to in vivo 31P metabolite quantitation, 1H MR imaging was conducted to identify brain tumor and to determine tumor volume prior to 31P MRS.