Abstract #2069

Brain Circuit Analysis with Real-Time Optogenetic Functional Magnetic Resonance Imaging (Rt-OfMRI)

Zhongnan Fang¹, Jin Hyung Lee¹,²

¹Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, CA, United States; ²Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, Los Angeles, CA, United States

Here we present a novel real-time optogenetic functional magnetic resonance imaging (ofMRI) method. ofMRI is a novel technology, which enables systematic analysis of brain circuits by using optogenetic stimulation as input, and fMRI readout as output. Real-time ofMRI (rt-ofMRI) aims to provide real-time brain circuit analysis capabilities using ofMRI. Our goal is to acquire, reconstruct, motion correct, and analyze ofMRI images in real-time. This approach enables high-throughput ofMRI studies with live activation feedback on image quality, motion characteristics, and optogenetic stimulation response. It prevents long offline processing and facilitates interactive experiment parameter selection (e.g. stimulation frequency, isoflurane level, etc.).