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Abstract #2530

Fast Gated EPR Imaging of the Beating Heart: Spatiotemporally-Resolved 3D Imaging of Free Radical Distribution During the Cardiac Cycle

Zhiyu Chen1, Levy A. Reyes1, David H. Johnson1, Murugesan Velayutham1, Changjun Yang1, Alexandre Samouilov1, Jay L. Zweier1

1Davis Heart and Lung Research Institute, Ohio State University Medical Center, Columbus, OH, United States

In Vivo or ex vivo electron paramagnetic resonance imaging (EPRI) is a powerful technique for determining the spatial distribution of free radicals and other paramagnetic species in living organs and tissues. However, applications of EPRI have been limited by long projection acquisition times and the consequent fact that rapid gated EPRI was not possible. Hence in vivo EPRI typically provided only time-averaged information. In order to achieve direct gated EPRI, a fast EPR acquisition scheme was developed to decrease EPR projection acquisition time down to 10 20 ms, along with corresponding software and instrumentation to achieve fast gated EPRI of the beating heart with submillimeter spatial resolution in as little as 2 to 3 minutes. Reconstructed images display the temporal and spatial variations of the free radical distribution, anatomical structure, and contractile function of a rat heart during the cardiac cycle.