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Abstract #3535

Pharmacological MRI in Mice with the Rapid Steady State T1-Technique and Intraperitoneal Contrast Agent Administration

Teodora-Adriana Perles-Barbacaru1, Francois Berger1, Hana Lahrech1

1Grenoble Institute of Neurosciences, INSERM U836, Grenoble, France

The Rapid Steady State T1 (RSST1) MRI technique, previously used with intravenous injections, can be used with intraperitoneal (i.p.) injections of Gd-DOTA to acquire cerebral blood volume fraction (BVf) maps in mice. The longer time window after i.p. administration of Gd-DOTA is used to study the sensitivity of the RSST1-technique to BVf changes induced by the vasodilator Acetazolamide. A 20% BVf increase was observed within 10 minutes after Acetazolamide injection confirming the vascular biodistribution of Gd-DOTA and validating the technique. The RSST1-technique with i.p. administration of Gd-DOTA can therefore be used for pharmacological MRI experiments in mice.