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Abstract #4315

Effects of Tumour Oxygenation on 13C Pyruvate Metabolism

Steven Reynolds1, Samira Kazan2, Joanne Bluff2, Emily Wholey2, Martyn Paley1, Gillian Tozer2

1Academic Unit of Radiology, University of Sheffield, Sheffield, South Yorkshire, United Kingdom; 2Tumour microcirculation group, University of Sheffield, United Kingdom

The influence of tumour oxygenation state was determined by administering hyperpolarised 13C1-pyruvic acid (PA) and determining the conversion rate, kpl, to lactate. The tumours oxygenation state was concurrently monitored during MR experiments using invasive Oxylite fluorescent probes. P22 carcinosarcoma-bearing BD1X rats were anaesthetised and femoral cannulations performed for drug administration/blood pressure monitoring. Tumour pO2 was manipulated by supplying either normal air or hypoxia (10% O2, 4% CO2, balance N2). 5ml/kg of hyperpolarised 13C-PA was injected and slice-localised 13C spectroscopic data acquired using a 20mm 13C, 1H surface coil positioned over the tumour in a Bruker 7T MRI system Integral versus time responses curves for pyruvate and lactate were fitted to a one-way exchange model and kpl values extracted. Date for n=6 animals showed that rate of conversion of pyruvate to lactate, kpl, increases under hypoxic conditions.