Abstract #4414

Proton NMR Metabolomics and Genomics Show Induction of Insulin Resistance in Murine Skeletal Muscle in Response to Treatment with a Small Volatile Bacterial Molecule

Valeria Righi¹, Caterina Constantinou², Nikolaos Psychogios³, Julie Wilhelmy⁴, Michael Mindrinos⁴, Laurence G. Rahme², Aria A. Tzika³

¹NMR Surgical Laboratory, Department of Surgery , Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston , MA, United States; ²Molecular Surgery Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burn Institute, Harvard Medical School, Boston, MA, United States; ³NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burns Institute, Harvard Medical School, Boston, MA, United States; ⁴Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, United States

We applied HRMAS 1H NMR metabolomics to intact muscle samples from animals treated with a volatile aromatic low molecular weight molecule, 2-AA, in order to identify at high-resolution concomitant metabolic and molecular aberrations associated with insulin resistance and metabolic dysfunction. These changes might also reflect mitochondrial dysfunction, as some of the genes downregulated in response to 2-AA treatment encode mitochondrial-dependent metabolic functions. The strength of HRMAS is that it allows dual investigation of metabolic and molecular changes since the same specimens studied with HRMAS can subsequently be used for transcriptome studies.