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Abstract #0815

Revealing the Hyperpolarized 129Xe Red Blood Cell Resonance Using Transgenic Mice

Matthew S. Freeman1, Zackary I. Cleveland2, Yi Qi2, Bastiaan Driehuys, 12

1Medical Physics Graduate Program, Duke University, Durham, NC, United States; 2Department of Radiology, Duke University, Durham, NC, United States

Hyperpolarized 129Xe provides a unique means of probing gas exchange, being soluble in tissues and displaying a large in vivo chemical shift range. However, mice do not typically display a unique RBC resonance, as rats and humans do. For the first time, transgenic mice expressing for human hemoglobin display two dissolved-phase NMR peaks at 198 and 220 ppm, almost identical to the interstitial tissue and RBC peaks seen in humans. This enables visualization of pulmonary gas-exchange not previously possible in mice, providing global and regional physiological information about gas transit to the RBCs in mouse models of disease and injury.