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Abstract #0862

Metabolic Flux Maps from 3D MRSI of Rat Brain in Situ

Sungtak Hong1, Golam M.I. Chowdhury2, Xiaomin Ma1, Monique Thomas2, Graeme F. Mason2, Gerard Sanacora2, Douglas L. Rothman1, Kevin L. Behar2, Robin A. de Graaf1

1Diagnostic Radiology, Yale University, New Haven, CT, United States; 2Psychiatry, Yale University, New Haven, CT, United States

Recently, a new ex vivo assay that combines 3D magnetic resonance spectroscopic imaging (MRSI) with focused-beam microwave-euthanasia was developed to generate quantitative metabolic maps of brain metabolites at high spatial resolution. In this work, detailed processing procedures and routines are described to analyze 3D MRSI data acquired from rat brain receiving timed intravenous infusions of 13C labeled glucose. The rich dynamic metabolic information obtained in the resulting metabolic maps is demonstrated.