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Abstract #0863

Oxygen-17 MRS for CMRO2 Measurements in the Mouse Brain at 16.4T

Weina Cui1, Xiao-Hong Zhu1, Manda Vollmers1, Emily Colonna1, Gregor Adriany1, Brandon Tramm2, Janet Dubinsky1, Gulin Oz1

1University of Minnesota, Minneapolis, MN, United States; 2Virtumed, LLC, Minneapolis, MN, United States

To assess cerebral energetics in transgenic mouse models of neurological disease, a robust and quick method for quantification of cerebral oxygen consumption is needed. 17O MRS methodology has been validated in rats and cats to measure CMRO2, however mice present unique challenges due to their small size. We show that 17O MRS in the mouse brain is feasible with high sensitivity using 16.4T, a surface 1H/17O coil and a newly designed oxygen delivery system. The method can be utilized to measure mitochondrial function in mice quickly and repeatedly, without oral intubation, and has numerous potential applications to study cerebral energetics.