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Abstract #1268

MEMRI Based NOD/scid-IL-2R&[gamma] cnull Mouse Brain Atlas for HIV Pathobiology Studies

Balasrinivasa R. Sajja1, Biyun Zhou2, Mariano G. Uberti1, Larisa Y. Poluektova3, Howard E. Gendelman3, Michael Douglas Boska1, 3, Yutong Liu1, 3

1Radiology, University of Nebraska Medical Center, Omaha, NE, United States; 2Anesthesiology, Tongji Medical College, Huanzhong University of Science and Technology, Wuhan, China; 3Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, United States

Strain specific mouse brain MR atlases provide a coordinate space for spatial normalization and permit longitudinal quantitative analyses associated with aging and disease progression. Manganese enhanced MRI (MEMRI) enhances anatomic structures in the NOD/scid-IL-2R&[gamma] cnull (NSG) mice. NSG mice can propagate human cell transplants and thus be used to study human disease. Image post-processing based on Laplacian operator was used to enhance brain structures boundaries without introducing artifacts. Thus, an in vivo MEMRI based NSG mouse brain atlas was generated. This atlas will be used to track structure-wise alterations during progression of HIV-1 in NSG mice implanted with human immune cells.