Abstract #1584

in vivo Imaging of Free Radicals in Cardiac Tissues from Diabetic Mice with the Use of MRI

Rheal A. Towner¹, Nataliya Smith¹, Debra Saunders¹, Jorge Carrizales¹, Florea Lupu², Robert Silasi-Mansat², Kenneth Humphries³, Shraddha Vadvakar³, Marilyn I. Ehrenshaft⁴, Ronald P. Mason⁴

¹Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; ²Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; ³Free Radical Biology & Medicine, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; ⁴Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States

Free radicals have been shown to play a major role in the pathogenesis associated with cardiac problems in diabetes. This study reports on in vivo imaging of membrane bound radicals (MBR) with the use of molecular MRI (mMRI) and immuno-spin trapping (IST) on diabetic cardiac muscle in a streptozotocin (STZ)-induced model. The spin trapping compound DMPO (5,5-dimethyl-pyrroline-N-oxide) is used to trap radicals that can be recognized by an anti-DMPO probe with an albumin-anti-DMPO-antibody-Gd-DTPA-biotin construct. This method can be used to understand the role of free radicals in cardiac-related pathogenesis in diabetes.