Rheal A. Towner1, Nataliya Smith1, Debra Saunders1, Jorge Carrizales1, Florea Lupu2, Robert Silasi-Mansat2, Kenneth Humphries3, Shraddha Vadvakar3, Marilyn I. Ehrenshaft4, Ronald P. Mason4
1Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 2Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 3Free Radical Biology & Medicine, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 4Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States
Free radicals have been shown to play a major role in the pathogenesis associated with cardiac problems in diabetes. This study reports on in vivo imaging of membrane bound radicals (MBR) with the use of molecular MRI (mMRI) and immuno-spin trapping (IST) on diabetic cardiac muscle in a streptozotocin (STZ)-induced model. The spin trapping compound DMPO (5,5-dimethyl-pyrroline-N-oxide) is used to trap radicals that can be recognized by an anti-DMPO probe with an albumin-anti-DMPO-antibody-Gd-DTPA-biotin construct. This method can be used to understand the role of free radicals in cardiac-related pathogenesis in diabetes.