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Abstract #1859

T Cell Labeling and Tracking by MRI in Mouse Brain During Viral Infection

Tatjana Atanasijevic1, Jasmin Herz1, Dorian McGavern1, Alan P. Koretsky1

1NINDS, NIH, Bethesda, MD, United States

Viral infection of the central nervous system (CNS) is a common cause of inflammatory disease. Anti-viral T cells recruited from the periphery play a critical role in fighting CNS viral infections. The ability to track single T cells in the brain with MRI would help pre-clinical studies and open novel diagnostic possibilities. Here it is demonstrated that T cells can be labeled with MPIOs in short incubation times and sorted for cells that have internalized particles. These cells can be re-introduced into LCMV-infected mice and detected by MRI opening the possibility of tracking single T cells migrating in the brain.