Rheal A. Towner1, Nataliya Smith1, Philippe Garteiser1, Debra Saunders1, Florea Lupu2, Robert Silasi-Mansat2, Dario C. Ramirez3, Sandra E. Gomez-Mejiba3, Ronald P. Mason4, Marilyn I. Ehrenshaft4, Fernando Bozza5, Marcus Frenandes de Oliveira6, Hugo C. Castro Faria-Neto7
1Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 2Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 3Laboratory of Experimental and Therapeutic Medicine, National University of San Luis, San Luis, Argentina; 4Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States; 5Instituto de Pesquisa Clinica Evandro Chagas, Fundao Oswaldo Cruz, Rio de Janeiro, RJ, Brazil; 6Instituto de Bioqumica Mdica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil; 7Instituto de Pesquisa Clinica Evandro Chagas, Fundao Oswaldo Cruz, Rio de Janeiro, Brazil
Oxidative stress from free radicals plays a major role in sepsis. A combination of immuno-spin-trapping (IST) and targeted molecular magnetic resonance imaging (mMRI) was used to detect in vivo levels of membrane-bound radicals (MBR) in brain tissues from mice with cecal ligation puncture (CLP)-induced sepsis. The spin trapping compound DMPO (5,5-dimethyl pyrroline N-oxide) was used to trap radicals. An anti-DMPO probe (anti-DMPO antibody covalently bound to an albumin-Gd-DTPA-biotin construct was used to detect MBR in vivo with mMRI. This method can be applied towards any radical-associated pathological condition for the in vivo assessment of membrane-bound protein and/or lipid radical levels.