Rheal A. Towner1,
Nataliya Smith1, Debra Saunders1, Patricia Coutinho De
Souza1, Leah Henry1, Florea Lupu2, Robert
Silasi-Mansat2, Marilyn I. Ehrenshaft3, Ronald P. Mason3,
Sandra E. Gomez-Mejiba4, Dario C. Ramirez4
1Advanced
Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma
City, OK, United States; 2Cariovascular Biology, Oklahoma Medical
Research Foundation, Oklahoma City, OK, United States; 3Laboratory
of Pharmacology & Chemistry, National Institute of Environmental Health
Sciences, Research Triangle Park, NC, United States; 4Laboratory
of Experimental Medicine & Therapeutics, National University of San Luis,
San Luis, Argentina
Free radicals associated with oxidative stress play a major role in cancer. By combining immuno-spin-trapping (IST) and targeted molecular magnetic resonance imaging (mMRI), we detected in vivo levels of membrane-bound radicals (MBR) within tumors of GL261 glioma-bearing mice. The spin trapping agent DMPO (5,5-dimethyl pyrroline N-oxide) traps MBR which can be detected in vivo with a targeted molecular MRI probe that detects DMPO-trapped-MBR. This is the first report regarding the detection of in vivo levels of MBR from a glioma model. This novel non-invasive method can be applied to investigate free radical mechanisms in the pathogenesis of various cancers.
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