Eszter Boros1, Miloslav Polasek1, Zhaoda Zhang1, Peter Caravan2
1Department of Radiology, Massachusetts General Hospital, Harvard Medical School, The Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, MA, United States; 2Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Massachusetts General Hospital, Charlestown, MA, United States
We investigated applications of the alanine analogue of Gd(DOTA), Gd(DOTAla). Fmoc protected DOTAla suitable for solid phase peptide synthesis was synthesized and integrated into polypeptide structures containing 1-3 Gd(DOTAla) complexes (GdL1, GdL2, GdL3).GdL3 is superior to commercial contrast agents gadoteridol and gadofosveset /HSA (human serum albumin) at high fields. The modularity of design, the ease of solid phase synthesis, high kinetic inertness (t1/2 = 614h) for Gd3, and optimal water exchange rate renders the Gd(DOTAla) scaffold a suitable platform for the development of high field T1 agents based on Gd.