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Abstract #1957

In Vivo Determination of de Novo Lipogenesis in Rat Liver Using Localized 1H-[13C] Magnetic Resonance Spectroscopy

Sharon M. Janssens1, Klaas Nicolay1, Jeanine J. Prompers1

1Biomedical NMR, Eindhoven University of Technology, Eindhoven, Netherlands

De novo lipogenesis is the primary pathway in which excess carbohydrates are being converted to fat in liver. The aim of this study was to develop a non-invasive method for the direct in vivo measurement of de novo lipogenesis in rat liver using localized 1H MRS with 13C editing. Before and after the oral administration of [U-13C6] glucose for 5 days, total and 13C-enriched intrahepatocellular lipid (IHCL) content were measured with 1H-[13C] MRS. After 5 days of [U-13C6] glucose administration, total as well as 13C-enriched IHCL was increased, the latter being the result of de novo lipogenesis.