Angular Double-PGSE Spectroscopy of the Long Evans Shaker Spinal Cord

Debbie Anaby¹, Ian D. Duncan², Chelsey M. Smith², Yoram Cohen¹

Conventional single-pulsed-field-gradient (s-PFG) methodologies are capable of faithfully depicting diffusion anisotropy in coherently ordered structures. However, randomly oriented compartments are more difficult to characterize. Double-PFG (d-PFG) MR methodologies were recently suggested as an alternative for studying microstructure in CNS. Specifically, the angular d-PFG experiment may offer novel microstructural information that is not available from s-PFG by yielding specific signatures in relatively low q values. The angular d-PFG was used to distinguish between control and Long Evans Shaker (les) spinal cords by providing information on macroscopic anisotropy and compartment shape anisotropy.