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Abstract #2097

The Water Apparent Diffusion Coefficient, But Not T2, in ex vivo Brain Tissue Is Affected by Previous Exposure to Alkaline pH

Lindsey A. Leigland1, Marcus Cappiello2, Christopher D. Kroenke1, 3

1Advanced Imaging Research Center and Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR, United States; 2Department of Physics, Oregon State University, Corvallis, OR, United States; 3Division of Neuroscience, Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, United States

The goal of this research was to improve understanding of the determinants of physical properties (spin relaxation, diffusion, etc.) in postmortem tissue, as well as further characterize the dependence of these molecular properties on common laboratory tissue processing procedures. Here we show that exposure of tissue to alkaline conditions can induce dramatic (>2-fold) and irreversible changes in the water apparent diffusion coefficient (ADC) without affecting the water 1H transverse relaxation, an effect that is potentially realized through modifications to biological membranes. This research provides an avenue toward developing further insight into the determinants of physical parameters observable by MRI.