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Abstract #3690

ASL-Based Cerebral Perfusion with or Without Permanent Unilateral Common Carotid Artery Occlusion: Towards an Optimized Mouse Stroke Model.

Tom Dresselaers1, Wouter Oosterlinck2, Tom Struys1, 3, Kristof Govaerts1, Ivo Lambrichts3, Paul Herijgers2, Uwe Himmelreich1

1Department of Imaging & Pathology, KU Leuven, Leuven, Vl.Brabant, Belgium; 2Department of Experimental Cardiac Surgery, KU Leuven, Leuven, Vl.Brabant, Belgium; 3Department of Functional Morphology, Hasselt University, Diepenbeek, Limburg, Belgium

Unilateral Ligation of the common carotid artery (CCA) is usually performed for mouse stroke models. This study shows the impact of such a ligation on the cerebral blood flow using arterial spin labeling in sham operated animals under isoflurane anesthesia. Such ligation of the CCA may impact stroke development at early and later stages in the tMCAO mouse model. We demonstrate that a suture of the CCA avoids interhemispheric differences in CBF. We therefore speculate that this sutured tMCAO model may result in more consistent lesions, less mortality and a more realistic stroke model.